![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3589110:Design
Designed by: Yannik Schermer Group: iGEM20_TU_Kaiserslautern (2020-10-23)
Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii, Part 2 (Phytobrick)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 57
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 57
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 575
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 57
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 57
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The natural secretion signal of the marine bacteria was removed (?). This part is codon-optimized for Escherichia coli.
Source
This part originates from an uncultured marine bacteria (Yang, Q., 2018) and is condon-optimized for Escherichia coli.